To succeed in biomedical implant surgery, materials must be biocompatible, Willemite nanoparticles can be used in biomedical applications. MIT assay shows that willemite nanoparticles have non-toxic interaction between Hela (Cervical cancer) cell lines and the willemite found to be biocompatible for further applications in-veto systems. In depth cell particle interaction was carried out, by using staining technique coupled with inverted microscopy. The antibacterial test was performed against Gram-negative Escherickie colt (E.coli, ATCC 8739) and Gram-positive Staphylococcus aureur (S.aureus, ATCC 6538). The optimal properties with excellent antibacterial ability can be achieved when willemite nanoparticles concentration is between 0.30 ppm and 2.3 ppm. According to the obtained results, the synthesized nano composite powder confirms biocom-patibility of willemite, which could be an attractive candidate for biomedical applications Method: Pure Willemite Nanoparticles were synthesized via the modified sol-gel method. Synthe-sized powder was studied by Thermo gravimetric analysis (TGA), X-ray diffraction (XRD), scanning electron microscopy (SEM), Energy dispersive X-ray spectroscopy EDS, and transmission electron microscopy (TEM) with SAED techniques, The in vitro biocompatibility of willemite was studied. The biocompatibility of the Willemite nanoparticles was studied using Hela (Cervical cancer) cell lines with M’TT assays up to 24 h. _Result: Willemite nanoparticles were synthesized by facile sol-gel method. The synthesis method is cost-effective and easy to scale up. The results of this work demonstrate the applicability of wil-lemite nanoparticles in the biomedical field. The antibacterial rate increased from 65% to 99% while the cell viability decreased from 98% to 70 % when the willemite nanoparticles concentration varied from 0.05 to 190 ppm. Furthermore, the cytotoxicity studies show that the ivillemite has lower cyto-toxicity. Hence, it is revealed that willemite nano crystals are potentially applicable as bone substitu-tion materials in tissue engineering. Conclusion: Willemite nanoparticles possess effective in vitro noncytotoxicity and antibacterial activity. The favourable properties with excellent cytocompatibility and antibacterial ability can be achieved when willemite concentration is between 0.30 ppm and 2.3 ppm. The antibacterial wil-lemite is non-toxic to the living cells and tissues even if the particles are internalized by cells, Wil-lemite is an excellent candidate in the biomedical field. Novel willemite nano crystals may provide new opportunities for a non-cytotoxic implant with antibacterial ability in bone tissue engineering.
